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Spatial comparison of total vs. active bacterial populations by coupling genetic fingerprinting and clone library analyses in the NW Mediterranean Sea ArchiMer
Rodriguez-blanco, Arturo; Ghiglione, Jean-francois; Catala, Philippe; Casamayor, Emilio O.; Lebaron, Philippe.
Spatial distributions of both total (i.e. 16S rDNA-based fingerprints) and active (i.e. 16S rRNA-based fingerprints) bacterial populations, together with total bacterial activity measured by (3)H-leucine incorporation, were studied along a 98 km transect in the NW Mediterranean Sea. Capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) fingerprinting was coupled to a clone library, allowing CE-SSCP peaks identification and the monitoring of the spatial variation of bacterial phylotypes. Up to 80% of the community peaks matched those obtained from clone library sequences, accounting for 86.7% of the total fingerprinting area. A good agreement was found between the relative abundance of Prochlorococcus in the CE-SSCP fingerprints and...
Tipo: Text Palavras-chave: CE-SSCP fingerprinting; 16S rRNA; Spatial distribution; Bacterial diversity and activity Prochlorococcus; SAR11; Gammaproteobacteria.
Ano: 2009 URL: https://archimer.ifremer.fr/doc/00243/35433/33965.pdf
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